Genetic engineering & bioinformatics tools: Applications and methodologies in modern life sciences

This book provides a comprehensive introduction to the fundamental tools and techniques of genetic engineering and bioinformatics, bridging laboratory-based molecular biology with computational analysis. It covers core experimental methods such as nucleic acid extraction and purification from diverse biological sources, molecular hybridization techniques, nucleic acid amplification including PCR and its variants, and classical as well as next-generation DNA sequencing technologies. The book then extends these experimental foundations into bioinformatics, explaining key concepts, databases, analytical tools, and applications used to interpret large-scale genomic and omics data. Emphasis is placed on practical principles, methodological comparisons, and real-world applications in biology, medicine, agriculture, and biotechnology, making the book a practical guide for students and researchers seeking to understand both wet-lab techniques and data-driven biological analysis.