Title: Golden Gate Cloning : Methods and Protocols
Publisher: Humana Okt 2024
Publication Date: 2024
Language: English
ISBN 10: 1071640941
ISBN 13: 9781071640944
Binding: Buch
Condition: Neu
Dimensions: 260 millimeters width by 183 millimeters height by 34 millimeters depth
Weight: 1163 grams

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Synopsis

This volume provides an overview of the latest protocols that cover the full range of in silico, in vitro, and in vivo approaches to help researchers plan, design, and perform Golden Gate cloning experiments. The chapters in this book cover topics such as Golden Gate cloning of standardized parts; modular DNA construct design for high-throughput Golden Gate Assembly; Golden Gate cloning of Synthetic CRISPR RNA spacer sequences; Golden Gate cloning in Actinobacteria; and YeastFab cloning of toxic genes and protein expression optimization in yeast. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Golden Gate Cloning: Methods and Protocols is a valuable resource for all researchers interested in learning more about this important and developing field.

Chapter 3 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

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