Green Fluorescent Protein: Properties, Applications, and Protocols

Green Fluorescent Protein: Properties, Applications, and Protocols is the first book devoted to this rapidly evolving reporter system. This collection of essays from pioneers in the field tackles both theory and practice, offering numerous case studies, examples, illustrations, and troubleshooting tips. The book clearly demonstrates how to tailor GFP to specific systems, maximize expression and enhance detection in a variety of organisms and cell types, generate variants with altered properties, explore new ways to use GFP, and more. Topics covered include:
* The discovery of GFP
* Bioluminescence and biofluorescence in nature
* Biochemical and physical properties of GFP
* The three-dimensional structure of GFP and its implications for function and design
* Molecular biology and modification of GFP
* Uses of GFP in various organisms, including prokaryotes, yeast, C. elegans, drosophila, plants, transgenic vertebrates, and mammalian cells
* Detailed protocols for the expression of GFP, specimen preparation, visualization and recording of GFP fluorescence, and GFP websites

Edited by Martin Chalfie and Steven Kain

Green fluorescent protein (GFP), a biological marker cloned from jellyfish, has emerged from virtual obscurity into the forefront of biomolecular research in just a few years. Intensely studied in hundreds of in vivo projects worldwide, and resulting in new articles and applications at a breathtaking rate, GFP may well become one of the most powerful tools in molecular and cellular biology.

Green Fluorescent Protein: Properties, Applications, and Protocols is the first book devoted to this rapidly evolving reporter system. This collection of essays from pioneers in the field tackles both theory and practice, offering numerous case studies, examples, illustrations, and troubleshooting tips. The book clearly demonstrates how to tailor GFP to specific systems, maximize expression and enhance detection in a variety of organisms and cell types, generate variants with altered properties, explore new ways to use GFP, and more. Topics covered include:
* The discovery of GFP
* Bioluminescence and biofluorescence in nature
* Biochemical and physical properties of GFP
* The three-dimensional structure of GFP and its implications for function and design
* Molecular biology and modification of GFP
* Uses of GFP in various organisms, including prokaryotes, yeast, C. elegans, drosophila, plants, transgenic vertebrates, and mammalian cells
* Detailed protocols for the expression of GFP, specimen preparation, visualization and recording of GFP fluorescence, and GFP websites.

Green Fluorescent Protein: Properties, Applications, and Protocols lays the foundation for a new understanding of dynamic biological phenomena. It is essential reading for researchers and graduate students in molecular biology, physiology, biochemistry, cell and developmental biology, neuroscience, ecology, in vivo biology, and plant molecular biology.