Green Fluorescent Protein: Properties, Applications and Protocols (Methods of Biochemical Analysis)

Green Fluorescent Protein: Properties, Applications and Protocols, 2nd Edition

Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications.

This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.

MARTIN CHALFIE, PhD, is William R. Kenan Jr. Professor of Biological Sciences at Columbia University. He is world renowned for introducing the use of GFP as a biological marker. A member of the National Academy of Sciences, Dr. Chalfie serves on the editorial boards of Genome Biology and Molecular Biology of the Cell.

STEVEN R. KAIN, PhD, manages the Applications Gene Expression Group for Agilent Technologies. While director of cell biology at Clontech Laboratories, he was responsible for commercializing the first products based on GFP and produced the popular EGFP variant. He has twelve years' experience with a variety of biotechnology firms in the San Francisco Bay Area.