Guide to Techniques in Mouse Development, Part B: Mouse Molecular Genetics (Methods in Enzymology)

Guide to Techniques in Mouse Development, Part B, is an authoritative guide to different methods used in enzymology, focusing on investigating mouse development using technological advances. The text provides information regarding the principles of the methods in mouse development, and it offers readers reliable experimental protocols and recipes described comprehensively by leaders in the field of enzymology.

The text is divided into three sections and organized into 25 chapters. Below are several concepts covered by the text:

• Lentivirus transgenesis o Germline modification using mouse stem cells
• Electroporation
• Applications of transposons in mouse genetics
• Functional genomics using transposon systems
• The use of DNA transposons in detecting cancer genes in mice
• Recombination, conditional mutagenesis and induction of tamoxifen
• Genetic fate mapping using recombinases
• Genetic screens mouse ES cells
• Gene trap mutagenesis
• Mouse mutagenesis
• Self- renewal and pluripotency
• Transgenic RNAi applications
• Gene knockdowns
• Tetracycline-controlled transcription
• Gene expression profiling of mouse embryos

The book is a comprehensive guide for students and professionals in genetics, cytology and molecular biology, who will find this book invaluable for their learning and practice.

• Update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993
• Comprehensively covers:
• new techniques for the cryopreservation of gametes and embryos
• production of transgenic and null (knockout) animals (use of ES cells)
• generation of conditional/inducible mutant animals
• use of gene-trap mutagenesis
• analysis of allele-specific expression
• use of new reporter constructs
• humanizing of transgenic animals
• transcript profiling of mouse development
• imaging of mouse development
• rederivation of animals and use of mouse genomics

Philippe Soriano,is Professor of Cell, Developmental & Regenerative Biology and Professor of Oncological Sciences, Icahn School of Medicine at Mount Sinai, NY, USA