HPLC, a Practical User's Guide

HPLC: A Practical User's Guide is essential for chromatographers needing a proven training manual for setting up and running an HPLC system, for optimizing and cleaning columns and separations, and for obtaining separations and maximizing information from their system.

Relying upon 15 years of laboratory and field troubleshooting experience, the author explains the basic techniques and shows how to use them to optimize separations. Proven methods are provided for starting up and maintaining HPLC systems, cleaning and troubleshooting column to the system problems, and systematic column treatment and methods development, both manual and automated.

Coverage includes preparative HPLC, Diode Array, UV detectors, recent advances in HPLC/mass spectrometry, and a discussion of hardware configurations and interfacing to computers and data acquisition devices. A guide to separations, a glossary of terms, and a programmed self-learning guide are also included.

Everything you need to set up an HPLC systemthat takes advantage of the latest innovations

Here is the perfect handbook for both novice and experienced chromatographers. For novices, HPLC, Second Edition cuts through the technical detail, theory, and confusing variety of column types, enabling them to easily set up and maintain an HPLC system. For more experienced practitioners, the book explains how to fully exploit the most recent innovations and improvements in column technology. The latest information and techniques in high-temperature, micro-flow, and ultra-fast chromatography, as well as the linking of an HPLC to a mass spectrometer, represent just some of the new material featured in this Second Edition.

Readers benefit from the author's twenty-five years of experience in HPLC, first as a user, then as a field sales and application support representative, and finally as a teacher and consultant. The book's wealth of tips and troubleshooting advice originate from the author's own laboratory and field work.

HPLC's structure is carefully designed to give readers a solid foundationin the basics and then methodically build and refine their skills:

• Part I, HPLC Primer, presents the essentials needed to get an HPLC system up and running
• Part II, HPLC Optimization, explains how to make the best use of the common columns and troubleshoot problems
• Part III, HPLC Utilization, focuses on real-world applications, including interfacing HPLC systems with computers and robotic workstations

A CD-ROM with the author's PowerPoint® presentation, Practical Course in HPLC, is included with the book. Plus, new appendices offer answers to frequently asked questions, tables of solvents and volatile buffers, an updated glossary of terms, and laboratory experiments. In short, everything that you need to know to confidently separate, identify, purify, and quantify compounds is here.