HPLC-UV Method development for Fixed Dose Pharmaceutical Formulations | Analytical Method Development and Validation for selected Fixed-Dose Formulations Using HPLC and UV Spectroscopy | Sonia Yadav (u. a.) | Taschenbuch | Englisch | 2026 | LAP LAMBERT Academic Publishing | EAN 9786209779718 | Verantwortliche Person f�r die EU: SIA OmniScriptum Publishing, Brivibas Gatve 197, 1039 RIGA, LETTLAND, customerservice[at]vdm-vsg[dot]de | Anbieter: preigu.

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Title

HPLC-UV Method development for Fixed Dose Pharmaceutical Formulations | Analytical Method Development and Validation for selected Fixed-Dose Formulations Using HPLC and UV Spectroscopy

Publisher

LAP LAMBERT Academic Publishing

Publication Date

2026

Language

English

ISBN 10

6209779719

ISBN 13

9786209779718

Binding

Taschenbuch

Condition

Neu

Dimensions

Weight

96 grams

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Synopsis

Estimation of imeglimin hydrochloride in human plasma by HPLC-UV. The estimation of drugs by RP-HPLC (reverse phase chromatography) was chosen since it is advised for use with ionic and mild to non-polar compounds. Simple, specific, and superior in terms of efficiency, stability, and reproducibility is reverse phase chromatography. For the separation of imeglimin, the C18 column 250 x 4.6 mm, 5 �m particle size was chosen. In the mobile phase, various solvent solutions were tested and combined for optimum performance. Imeglimin hydrochloride (4 �g/ml) in buffer, pH 4 for Acetonitrile: Water (70:30), a concentration range (8-40 and 0.5-4 �g/ml) displaying excellent peak and a sizable resolution level. Having the mobile phase run at 1ml/min, photodiode array detectors were used to detect both analytes at 215 nm. The method was validated in different concentration ranges in human plasma. Imeglimin inter and intra-run precision was measured at less than 3.60% and had an accuracy of less than 1.869%. imeglimin in human plasma was validated by linearity, recovery matrix, and stability. The retention time of IMEG is 4.8 minutes in plasma. Suggested method denotes that it is cost-effective

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