Title: Haploid technology- A potential tool for crop improvement in Rice: Development of double haploids in indica Rice genotypes via in vitro anther culture
Publisher: LAP LAMBERT Academic Publishing
Publication Date: 2017
Language: English
ISBN 10: 3330081066
ISBN 13: 9783330081062
Binding: Soft cover
Condition: New

About this title

Synopsis

Plant tissue culture techniques are recognized as useful instruments in crop improvement. Among these haploid technology is one in which haploid embryos are regenerated from male and female gametes for the production of haploid and double haploid plants who are homozygous in origin. In this technique, in vitro anther culture stands out and is an increasingly powerful tool when integrated with breeding programs as it results in pure lines. To develop a genotype specific methodology for double haploidy 8F1, 1F2 and 1F3 rice genotypes have been obtained from NSL. The focus of this project was to standardize an efficient protocol for double haploid production for all the genotypes, optimize media components especially hormones and cold stress to induce direct embryogenesis, plant regeneration and chromosome doubling in haploid regenerants to get double haploids. From our work we have developed some double haploid lines which are far superior to the hybrid parent. Some of the improved features are double haploid lines with high aroma, good cooking quality, high yielding, lodging tolerant and BLB and Blast resistant.

About the Author

Dr.ARJUN H.M, with Ph.D from the department of biotechnology, JNTU Hyderabad, India. With experience of about 14 years in the field of plant biotechnology and specialized in plant tissue culture- anther, microspore culture and agrobacterium mediated plant transformation. Currently working as Principal Scientist at Nuziveedu Seeds Limited, Hyderabad

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