Heterologous Gene Expression in E.coli : Methods and Protocols

Language: English

Published by Humana Press, 2016

1493957252 / 9781493957255

Series: Book 74 of 382 - Methods in Molecular Biology

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Seller: AHA-BUCH GmbH, Einbeck, GermanyAHA-BUCH GmbH

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nach der Bestellung gedruckt Neuware - Printed after ordering - Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular Biology(TM) format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.…

Seller Inventory # 9781493957255

Title
Heterologous Gene Expression in E.coli : Methods and Protocols
Author
Ming-Qun Xu
Publisher
Humana Press
Publication year
2016
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
1493957252
ISBN 13
9781493957255
Item weight
612 grams
Dimensions
254x178x18 mm
Series
Book 74 of 382: Methods in Molecular Biology

AHA-BUCH GmbH

Einbeck, Germany

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