High-Throughput Imaging Methods

This comprehensive volume offers a cutting-edge examination of high-throughput and label-free analysis techniques, showcasing their transformative impact on biomedical, pharmaceutical, and materials research. By bridging historical context with the latest technological advances, the book provides a detailed overview of how high-throughput screening (HTS)―once limited to synthetic compound assays―has evolved into a powerful, label-free platform capable of probing biological phenomena at the single-cell and single-molecule level. The topics span from surface plasmon resonance, hyperspectral imaging, and single-molecule electronics to regenerative tissue engineering and organoid development, culminating in discussions on soft, bio-integrated electronics for real-time monitoring. Each chapter integrates fundamental principles with applied research, providing readers with a panoramic view of how HTS technologies are being redefined across various domains. The book serves as a crucial reference for the next generation of bioanalytical and nanotechnology tools driving innovation in diagnostics, drug discovery, and tissue engineering and is ideal for researchers, industry scientists, graduate students, and anyone eager to engage with this rapidly evolving field.

This book provides a unified resource on emerging label-free and high-throughput techniques, which are crucial for next-gen diagnostics, drug development, and tissue modeling. It bridges the gap between traditional assays and modern, real-time, non-invasive analyses that are critical for precision medicine.

Manas Ranjan Gartia is an associate professor of mechanical and industrial engineering at Louisiana State University (LSU), Baton Rouge, Louisiana, USA, and a fellow of the US National Academy of Inventors and the Royal Society of Chemistry. He earned his PhD in mechanical engineering from the University of Illinois at Urbana-Champaign (2013). He is especially recognized for label-free biosensing technologies and for his significant contributions to cancer diagnostics and metabolomic imaging. Dr Gartia’s work exemplifies how engineering innovations can directly transform life sciences and healthcare applications. His research focuses on biomedical imaging, spatial lipidomics, cancer biology, and biophotonics and biotechnology.

Ram V. Devireddy is the DeSoto Parish Chapter University alumni professor and the Louisiana Land & Exploration Company endowed chair professor of mechanical engineering at LSU. He earned his PhD in mechanical engineering from the University of Minnesota (1999). He is a fellow of the American Society of Mechanical Engineers and is widely recognized for his contributions to biothermal engineering and bioprinting technologies. Dr Devireddy’s work bridges mechanical engineering principles with biological applications, making him a key figure in advancing tissue engineering and regenerative medicine. His research interests include heat and mass transport in biosystems, stem cell differentiation, tissue engineering, 3D bioprinting, and next-generation sequencing.