Histone Methyltransferases : Methods and Protocols

Daniel Holoch

ISBN 10: 1071624806 / ISBN 13: 9781071624807

Published by Springer US, Springer US, 2022

About this Item

Druck auf Anfrage Neuware - Printed after ordering - This volume provides methods used to investigate histone methyltransferase function. Chapters guide readers throughacomprehensive set of approaches that detailphylogenetic diversity, histone demethylase activitiesin vitro,generating chromatin substrates, auto-methylation, quantification of metabolites, protein purification, crystallization, X-ray structure, cryogenic electron microscopy,assessinggenome-wide patterns, CUT&Tag in mouse embryonic tissues, chemical biology approaches, peptide SPOT arrays, nascent chromatin capture, ectopic protein tethering, computational models, anddevelopment of methyltransferase inhibitors.Written in the format of the highly successfulMethods in Molecular Biologyseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge,Histone Methyltransferases: Methods and Protocolsaimsto be auseful and practical guide to new researchersand experts looking to expand their knowledge.

Seller Inventory # 9781071624807

Bibliographic Details

• Title: Histone Methyltransferases : Methods and Protocols
• Publisher: Springer US, Springer US
• Publication Date: 2022
• Language: English
• ISBN 10: 1071624806
• ISBN 13: 9781071624807
• Binding: Buch
• Condition: Neu
• Dimensions: 260W x 183H x 34D millimeters 260 millimeters width by 183 millimeters height by 34 millimeters depth
• Weight: 1157 grams

About this title

Synopsis

This volume provides methods used to investigate histone methyltransferase function. Chapters guide readers through a comprehensive set of approaches that detail phylogenetic diversity, histone demethylase activities in vitro, generating chromatin substrates, auto-methylation, quantification of metabolites, protein purification, crystallization, X-ray structure, cryogenic electron microscopy, assessing genome-wide patterns, CUT&Tag in mouse embryonic tissues, chemical biology approaches, peptide SPOT arrays, nascent chromatin capture, ectopic protein tethering, computational models, and development of methyltransferase inhibitors. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Histone Methyltransferases: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.