Human Cell Culture Protocols

Language: English

Published by Humana Nov 2011, 2011

1617793663 / 9781617793660

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This item is printed on demand - Print on Demand Titel. Neuware -Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement. This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the in vitro use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 452 pp. Englisch.

Seller Inventory # 9781617793660

Title
Human Cell Culture Protocols
Author
Ragai R. Mitry
Publisher
Humana Nov 2011
Publication year
2011
Condition
Neu
Binding
Buch
Language
English
ISBN 10
1617793663
ISBN 13
9781617793660
Edition
3rd Edition
Item weight
1,041 grams
Dimensions
260x183x29 mm

buchversandmimpf2000

Emtmannsberg, BAYE, Germany

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