Pluripotent stem cells (PSC) can divide indefinitely, self-renew, and can differentiate to functionally reconstitute almost any cell in the normal developmental pathway, given the right conditions. This comprehensive book, which was developed from a training course, covers all of the PSCs (embryonic, embryonic germ, and embryonic carcinoma) and their functions. It demonstrates the feeder-dependent and feeder-free culture of hESC and hiPSC, which will be referred to in all protocols as PSCs. It also addresses the methods commonly used to determine pluripotency, as defined by self-renewal marker expression and differentiation potential.

Human Pluripotent Stem Cells: A Practical Guide offers in-depth chapter coverage of introduction to stem cell, PSC culture, reprogramming, differentiation, PSC characterization, and more. It also includes four appendixes containing information on reagents, medias, and solutions; common antibodies; consumable and equipment; and logs and forms.

Includes helpful tips and tricks that are normally omitted from regular research papers
Features useful images to support the technical aspects and results visually as well as diagrammatic illustrations
Presents specific sections (ie: reprogramming, differentiation) in a concise and easily digestible manner
Written by experts with extensive experience in stem cell technologies

Human Pluripotent Stem Cells: A Practical Guide is an ideal text for stem cell researchers, including principal investigators, and others in university and industry settings, and for new graduate students in PSC labs.

About the Author

Uma Lakshmipathy, Director R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA

Chad C. MacArthur, Staff Scientist R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA

Mahalakshmi Sridharan, Scientist R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA

Rene H. Quintanilla, Scientist R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA

"About this title" may belong to another edition of this title.

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