Immunochemistry 1: A Practical Approach (Practical Approach Series)

Immunochemistry is of immense importance to virtually all areas of modern biology and medicine. Immunochemistry I and its companion volume, Immunochemistry II, offer a comprehensive compendium of the latest techniques in immunochemistry in a clear and accessible format. Volume I details the current strategies and methods used in the production of high quality antibodies, including chemically and genetically engineered antibodies, human monoclonal antibodies from B lymphocytes, anti-tumor antibodies, and antibodies packaged in eggs. Other topics include the uses of enzymes and antibodies in immunoassays; the standardization of antigens and antibodies; the latest ELISA techniques including enzyme amplification systems; enzyme immunoassays for detecting cell-surface molecules, single cell products, and proliferation; photoluminescence immunoassays; the incorporation of antibodies and their fragments into immunotoxins; and the use of synthetic peptides in mapping the epitopes of both antibodies and T-cells.
Volume II discusses the measurements of immunologically relevant molecules; immunofluorescence and immunoenzyme histochemistry; the use of synthetic peptides and liposomes in immunology; the use of antibodies as analytical probes and separation tools in fluorescence, blotting, and affinity chromatography; the measurement of antibody affinity; solid-phase assays of avidity; confocal laser scanning microscopy; flow cytofluorimetry; analysis of adhesion molecules; assays for complement receptors and for complement components. Each chapter includes clear technical advice, hints and tips, and sample results to help insure practical success.

Alan P. Johnstone is at St George's Hospital Medical School, London. Malcolm W. Turner is at Institute of Child Health, London.