Immunology and Immunotechnology

Contents Part I. Immunology 1. History and scope of immunology. 2. Immunity. 3. Haematopoiesis. 4. Cells and organs of the immune system. 5. Immune responses. 6. Antigens. 7. Immunoglobulins. 8. Organization and expression of Ig genes. 9. Ag Ab reactions. 10. Complements. 11. Cytokines. 12. Major histocompatibility complex. 13. Antigen processing and presentation. 14. Immunological tolerance. 15. Hypersensitivity. 16. Transplantation. 17. Autoimmunity. 18. Immunodeficiency diseases. 19. Immune responses to infectious diseases. 20. Vaccines. 21. Tumor immunology. 22. Host parasite relationship. 23. Normal microflora. 24. Infection. Part II. Immunotechnology 1. Identification of blood cells. 2. Identification and enumeration of B and T cells. 3. Preparation of specimen for immunology. 4. Preparation of blood antigens. 5. Preparation of antigens from pathogenic bacteria. 6. Purification of antigen and antibody by immunoadorbents. 7. Purification of immunoglobulins. 8. Quantification of antigen/antibody. 9. Conjugation of fluorochromes to antibodies. 10. Tagging of enzymes to antibodies. 11. Isolation of monocytes/macrophages. 12. Macrophage culture. 13. Isolation of dendritic cells. 14. Nephelometry. 15. Fluorescence Activated Cell Sorting (FACS) analysis. 16. In Vivo methods of delayed type hypersensitivity assessment. 17. Lymphocyte activation. 18. Detection of immune complexes. 19. T cell cloning. 20. Detection of cryoglobulins and pyroglobulins. 21. Immunodiagnosis. 22. Immuno screening of recombinant library. 23. In Vitro and In Vivo immuno histochemical techniques. 24. Determination of cell viability. 25. Laboratory animals. 26. Purification and bioassay of cytokines. Terminologies/Glossary. Further readings. Index. Abbreviations. From the Preface This book provides a complete coverage of all the key topics in immunology and immunotechnology with colour illustrations. This book gives step by step detail on topics such as host parasite relationship normal microbial flora infection immunity immune responses hypersensitivity auto immune diseases etc. This text book is divided into two main sections immunology and immunotechnology. That division allows an integrated approach to the study of immunology and immunological techniques. The first section deals with all the basic aspects in immunology and each of the 24 chapters gives a deep description of the basic and main topics of immunology. The second part is an easy to use companion to current protocols in immunology immunologists in basic science and clinical work alike will find techniques they rely on including production purification of antigen and antibodies and nephelmetry FACS quantification of antibodies T cell cloning immuno screening of recombinant library immunodiagnosis and animal models. This text book is suitable for biological medical and veterinary courses that cover microbiology biotechnology bio informatics biochemistry biochemical technology zoology life sciences immunology and pathology as well as focused immunology courses. Readers are also asked to go through the basic topics like host parasite relationship normal microflora infection haematopoiesis before entering into main topics and compare with the given suitable illustrations. 328 pp.…