Immunology Methods Manual: The Comprehensive Sourcebook of Techniques (Volume 1)

The Immunology Methods Manual is a four-volume set comprising a comprehensive sourcebook of immunological methods and techniques. Unique in its project-oriented, rather than protocol-oriented approach, the emphasis is placed on describing the environment in which each method was developed and is used. The manual contains both advanced methods and descriptions and basic protocols. It is essential not only for those who study the immune system of man or mouse, but also those applying methods to other species. This new compilation of methods answers the needs of immunologists; molecular, developmental, and cell biologists; students and advanced researchers, and workers in clinical and diagnostic laboratories. Protocols are provided to guide day-to-day laboratory work as well as the development of long-term strategies. Vital contact information also encourages useful dialogue and interaction between authors and method-users.
More than 35 section editors and 350 authors have contributed to compile this four-volume practical guide to methods that work!

• Provides essential hands-on methods
• Presents protocols with background information
• Ideally suited for use in a laboratory
• Covers all molecular and cellular techniques

Key Features of the CD-ROM

• Compatible with Windows, Mac, and UNIX platforms
• Features a full range of advanced search capabilities
• Includes a personalized note creation feature enabling the user to add bookmarks and "post-its"
• Offers full cut, copy, and paste capabilities allowing printing, exporting, and editing
• Provides hyperlinks between solutions/reagents and their recipes; figures/tables and their text citations; and reference citations to their bibliographic listings
• Contains a Pathway feature which allows recording of content during a particular session or loading a pre-recorded tutorial
• Includes the animated film "The Immune System" by André Traunecker and Stefan Meyer

The Immunology Methods Manual is a fully comprehensive sourcebook of methods and techniques, published in 4 volumes. Unique in its project-oriented rather than protocol-oriented approach, the manual contains both advanced methods and descriptions of basic protocols. These have been meticulously compiled, and carefully presented to guide day-to-day laboratory work, as well as the development of long-term strategies. This 4-volume manual will be essential not only for those who study the immune systems of man or mouse, but also for those applying methods to other species. It will answer the needs of Immunologists, Molecular, Cell and Developmental Biologists, students and advance researchers, and workers in clinical and diagnostic laboratories for a comprehensive and practical guide. Vital contact information will also encourage useful dialogue and interactions between authors and method-users.
Led by Ivan Lefkovits, 5 internationally recognised Advisory Editors, over 35 expert Section Editors and over 350 authoritative and experienced authors have contributed to compile this practical guide to methods that work!
Contents of Volume 1
Sections
1 - Expression of Recombinant Proteins
2 - Engineered Molecules
3 - Gene Targeting
4 - Monitoring V(D)J Rearrangement
5 - The Art of Polymearase Chain Reaction (PCR)
6 - Transcriptional Gene Regulation in B and T Cells
7 - mRNA Expression
8 - Antigen, Antibody and Immune Complexes
Contents of Volume 2
Sections
9 - MHC Ligands and Peptide Binding
10 - Screening of T and B Cell Epitopes
11 - Development of Cells of the B Lineage
12 - Ex Vivo and In Vitro Methods for Studying B Lymphopoiesis in Mouse and Man
13 - Modulation of the Humoral Immune Response and its Measurement
14 - Signaling Pathways and Lymphocyte Cell Repertoires
Contents of Volume 3
Sections
15 - Immunohistological Tools
16 - Lymhocytes as Single Cells
17 - Lymphoid Lines as Models for Physiological Cell Death
18 - Leukocyte Migration and Adhesion
19 - Mast Cells
20 - Dendritic Cells of Mice and Humans
21 - Isolation of Lymphocytes from Non-Lymphoid Tissues
22 - Gnotobiological Models
23 - Interaction of Peptidoglycan-Polysaccharides from the Human Intestinal Flora Bacteria and the Immune System
24 - Rheumatology in Relation to Immune Response and Microbes
25 - Experimental Models of Organ-Specific Autoimmunity (Nervous System)
26 - Experimental Models of Autoimmune Diseases
Contents of Volume 4
Sections
27 - Immunodiagnosis of Human B Cell Malignancies
28 - Immune Responses Against VSV, LCMV, and Vaccinia Virus In Vivo and In Vitro
29 - HIV and the Immune Response
30 - In Vivo Techniques of Experimental Immunology in Sheep
31 - In Vitro Techniques of Experimental Immunology in Sheep
32 - Experimental Immunology in Chickens
33 - Cold-Blooded Vertebrates
34 - Leukocyte Markers and the CD Nomenclature