Immunomodulating Impact of Probiotic Bacteria in the Gut: using the example of gene expression of Interleukin-8 and Interleukin-17 in Caco-2 cells

The gut is the largest human organ where interactions with the environment take place. A special role plays commensal gut bacteria,also called as microbiota. The interaction among microbiota and the human immune system are crucial for the maintenance of human immune system. An imbalance of immune response leads to chronic inflammation and further to the development of some diseases. It is well known that kind of diet and special foods or nutrients have an effect on appearance and concentration of inflammatory mediators and may affect the symptoms of diseases on a beneficial as well as on a negative way. Probiotics are discussed as a functional food. The autor Anne Gnauck gives an overview about microbiota, the intestinal immune system and the interaction among both in the first part. In the second part she explains her research project. The aim of the study was to investigate the influence of probiotic bacteria on inflammatory mediator IL-8 and IL-17 as well as their effect on caspase-3 mediated apoptosis. IL-8 ELISA and gene expression of IL-8, IL-17 and CASP3 were analyzed in Caco-2 cells after treatment with probiotic bacteria and commensal gut bacteria.

Anne Gnauck, Mag.rer.nat.: Study of Nutritional Sciences with the focus on Nutrition and Environment at the University of Vienna/ Austria and one semester abroad in the master program Public Health Nutrition at the University of Kuopio/ Finland. She worked as diploma student in the epigenetic work group of Dr. Alexander G. Haslberger.