The Inflammasome | Methods and Protocols

Christine M. De Nardo (u. a.)

ISBN 10: 1493960113 / ISBN 13: 9781493960118

Published by Humana, 2016

About this Item

The Inflammasome | Methods and Protocols | Christine M. De Nardo (u. a.) | Taschenbuch | xi | Englisch | 2016 | Humana | EAN 9781493960118 | Verantwortliche Person für die EU: Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu.

Seller Inventory # 103477346

Bibliographic Details

• Title: The Inflammasome | Methods and Protocols
• Publisher: Humana
• Publication Date: 2016
• Language: English
• ISBN 10: 1493960113
• ISBN 13: 9781493960118
• Binding: Taschenbuch
• Condition: Neu
• Dimensions: 254W x 178H x 14D millimeters 254 millimeters width by 178 millimeters height by 14 millimeters depth
• Weight: 460 grams
• Seller catalogs:
  • Bücher

About this title

Synopsis

Inflammasomes have a seemingly simple architecture. A sensor molecule of the NLR or PYHIN protein family recruits the common adapter molecule apoptosis-associated speck-like protein containing a CARD (ASC). Even so, inflammasome activation is highly regulated at multiple steps and precise details of how inflammasomes are activated at the molecular level remain largely unknown. In The Inflammasome: Methods and Protocols, expert researchers in the field detail methods and protocols to study multiple aspects of inflammasome function. These methods include the generation of inflammasome stimuli, perturbance of cellular homeostasis, assessment of ASC speck formation, monitoring of caspase-1 activity and processing, activation of IL-1β cytokines, and analysis of inflammasome assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.