Integrin Protocols

Language: English

Published by Humana Press, Humana Press Aug 2013, 2013

1489943412 / 9781489943415

Series: Book 17 of 382 - Methods in Molecular Biology

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Seller: buchversandmimpf2000, Emtmannsberg, BAYE, Germanybuchversandmimpf2000

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This item is printed on demand - Print on Demand Titel. Neuware -It is now widely accepted that the extracellular matrix (ECM) is a key determinant of tissue-specific gene expression. Signals provided by ECM are transduced by integrins, a large and growing superfamily of transmembrane heterodimeric cell surface receptors that link the ECM to structural and fu- tional elements within the cell. A wide range of cellular phenotypes have been shown to be regulated by integrins, including growth, differentiation, mig- tion, invasion, angiogenesis, and apoptosis. Furthermore, abnormalities of integrin expression and function have been implicated in the etiology of va- ous pathologic conditions, including cardiovascular disease, inflammatory disorders, and cancer. Thus integrins have emerged as an important class of molecules with wide ranging implications for understanding basic biological processes. In Integrin Protocols we provide a wide-ranging collection of laboratory protocols intended to assist investigators interested in integrins in working productively with these molecules, in studying their expression, and in pot- tially manipulating that expression to define their role(s) in relevant biolo- cal models. Protocols are provided for the analysis of integrin expression both at the RNA and protein levels (Chaps. 2, 5, and 7). Delcommenne and Streuli describe procedures for making rat monoclonal antibodies specific for mouse integrins; Schneller et al. and Arap and Huang describe methods for western blotting of integrins and RT-PCR analysis. Protocols are included that cover the analysis of the functional properties of integrins (Chaps. 1, 3, 4, 8, and 9 through 11). Koivunen et al.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 312 pp. Englisch.…

Seller Inventory # 9781489943415

Title
Integrin Protocols
Author
Anthony R. Howlett
Publisher
Humana Press, Humana Press Aug 2013
Publication year
2013
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
1489943412
ISBN 13
9781489943415
Item weight
455 grams
Dimensions
229x152x17 mm
Series
Book 17 of 382: Methods in Molecular Biology

buchversandmimpf2000

Emtmannsberg, BAYE, Germany

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