An Introduction to Single Molecule Biophysics

Yuri L Lyubchenko

ISBN 10: 1439806942 / ISBN 13: 9781439806944

Published by CRC Press Nov 2017, 2017

About this Item

Neuware - This introductory book broadly covers the foundations, principles, and methodologies of single molecule fluorescence and force probe techniques as applied to the most important and timely biological problems, providing a first introduction to key concepts and highlights the advantages and pitfalls of each of these exciting emerging methods.

Seller Inventory # 9781439806944

Bibliographic Details

• Title: An Introduction to Single Molecule Biophysics
• Publisher: CRC Press Nov 2017
• Publication Date: 2017
• Language: English
• ISBN 10: 1439806942
• ISBN 13: 9781439806944
• Binding: Buch
• Condition: Neu
• Dimensions: 236W x 155H x 18D millimeters 236 millimeters width by 155 millimeters height by 18 millimeters depth
• Weight: 590 grams

About this title

Synopsis

This book gives an accessible, detailed overview on techniques of single molecule biophysics (SMB), showing how they are applied to numerous biological problems associated with understanding the molecular mechanisms of DNA replication, transcription, and translation, as well as functioning of molecular machines. It covers major single molecule imaging and probing techniques, highlighting key strengths and limitations of each method using recent examples. The chapters begin with a discussion of single molecule fluorescence techniques followed by an overview of the atomic force microscope and its use for direct time-lapse visualization of dynamics of molecular complexes at the nanoscale, as well as applications in measurements of interactions between molecules and mechanical properties of isolated molecules and their complexes. The next chapters address magnetic tweezers and optical tweezers, including instrumentation, fundamentals of operation, and applications. A final chapter turns to nanopore transport and nanopore-based DNA sequencing technology that will play a major role in next-generation genomics and healthcare applications.

About the Author

Yuri L. Lyubchenko is Professor of Pharmaceutical Sciences at the University of Nebraska Medical Center, Omaha, NE, USA. His research focuses on understanding fundamental mechanisms underlying health and disease, which are key to developing new and more effective diagnostics and medications. This primarily basic research allows him not only identify new drug targets for small molecule drugs, it also leads to development of the nanotools and methods to discover novel approaches for diagnostic, treatment, and disease prevention, and to more rapidly determine their efficacy at the molecular level.