Laboratory Experiments in Microbiology

Newly revised to correspond to all current undergraduate one-semester microbiology textbooks. This lab manual includes 57 experiments that demonstrate the broad spectrum of microbiology and is an ideal companion to Microbiology: An Introduction, Ninth Edition by Tortora, Funke, and Case. Microscopy: Use and Care of the Microscope, Examination of Living Microorganisms; Staining Methods, Preparation of Smears and Simple Staining, Negative Staining, Gram Staining, Acid-fast Staining, Structural Stains (Endospore, Capsule, Flagella), Morphologic Unknown; Cultivation of Bacteria: Microbes in the Environment, Transfer of Bacteria: Aseptic Techniques, Isolation of Bacteria by Dilution Technique, Special Media for Isolating Bacteria; Microbial Metabolism: Carbohydrate Catabolism, Fermentation, Protein Catabolism, Respiration, Rapid Identification Methods; Microbial Growth: Oxygen and the Growth of Bacteria, Determination of a Bacterial Growth Curve: The Role of Temperature, Biofilms; Control of Microbial Growth: Physical Methods of Control: Heat, Physical Methods of Control: Ultraviolet Radiation, Chemical Methods of Control: Disinfectants and Antiseptics, Chemical Methods of Control: Antimicrobial Drugs, Effectiveness of Hand Scrubbing; Microbial Genetics: Regulation of Gene Expression, Isolation of Bacterial Mutants, Transformation of Bacteria, DNA Fingerprinting, Genetic Engineering, Ames Test for Detecting Possible Chemical Carcinogens; The Microbial World: Unknown Identification and Bergey's Manual, Fungi: Yeasts, Fungi: Molds, Phototrophs: Algae and Cyanobacteria, Protozoa, VIRUSES, Isolation and Titration of Bacteriophages, Plant Viruses; Interaction of Microbe and Host: Epidemiology, Koch's Postulate, IMMUNOLOGY, Nonspecific Resistance, Blood Group Determination: Slide Agglutination, Agglutination Reactions: Microtiter Agglutination, ELISA Technique; Microorganisms and Disease: Bacteria of the Skin, Bacteria of the Respiratory Tract, Bacteria of the Mouth, Bacteria of the Gastrointestinal Tract, Bacteria of the Urogenital Tract, Identification of an Unknown from a Clinical Sample; Microbiology and the Environment: Microbes in Water: Multiple-Tube Technique, Microbes in Water: Membrane Filter Technique, Microbes in Food: Contamination, Microbes Used in the Production of Foods, Microbes in Soil: The Nitrogen and Sulfur Cycles, Microbes in Soil: Bioremediaton; Appendices: Pipetting, Dilution Techniques and Calculations, Use of the Spectrophotometer, Graphing, Use of the Dissecting Membrane, Use of the Membrane Filter, Electrophoresis, Keys to Bacteria. For all readers interested in microbiology.