Laboratory Manual for Biochemical Analysis

Contents:1. Determination of Moisture Content 2. Determination of Fat by Soxhlet Method 3. Extraction of Lipids by Chloroform-Methanol Method 4. Determination of Specific Gravity of Crude oil 5. Preparation of Fatty Acid Methyl Esters and Dimethylacetals from Lipids with Boron Trifluoride-Methanol (BF3-methanol) Method 6. Determination of Total Nitrogen by Kjeldahl Method 7. Determination of Total Soluble Nitrogen 8. Determination of Non-Protein Nitrogen 9. The Preparation of Ash Extract Solution 10. Determination of Acid Insoluble Ash Content 11. Determination of Crude Fibre in the Sample 12. Determination of Ash and Organic Matter13. Determination of Total Volatile Bases (TVB) and Trimethylamine(TMA) by Diffusion Technique 14. Estimation of Total Volatile Nitrogen (TVN) 15. Determination of Peroxide Value 16. Determination of Iodine Value of Fats and Oil 17. Determination of Saponification Value 18. Determination of Free Fatty Acids (AOAC, 1986) 19. Determination of Salt Content using the Chlorocounter 20. Qualitative Tests for Carbohydrates 21. Determination of gluten content 22. Extraction of Starch from Potato and Determination of Purity by DNS Method 23. Determination of Thiobarbituric Acid (TBA)Value 24. Qualitative Tests for Lipids 25. Qualitative Tests for Protein/Amino Acids 26. Estimation of Carbohydrate by Anthrone Method 27. Determination Of Carbohydrate (Phenol Sulphuric Acid Method) 28. Estimation of Glucose by Ortho-Toluidine Method 29. Estimation of Glucose by Oxidase Method 30. Estimation of Protein by Lowry’s Method 31. Estimation of Protein by Biuret Method 32. Estimation of Protein by Bradford Method 33. Estimation of A/G (Albumin/Globulin) Ratio 34. Estimation of Cholesterol - Zak’s Method 35. Estimation of Triglyceride-Acetyl Acetone Method 36. Estimation of Inorganic Phosphorus 37. Estimation of Hypoxanthine Estimation using Xanthine Oxidase 38. Histamine Assay – by HPLC 39. Estimation of Iron in Blood by Wong’s Method 40. Estimation of Blood Sugar by Folin-Wu’s Method 41. Extraction and Estimation of Casein from Milk 42. Isolation of Casein from Milk 43. Estimation of Lactose in Milk by Lane-Eynon Method 44. Determination of Pectin Substance 45. Determination of Vitamin C (Ascorbic Acid) 46. Determination of Carotene 47. Determination of DPPH Radical Scavenging Activity 48. Estimation of Bilirubin in Serum by Malloy and Evelyn Method 49. Estimation of Calcium by Clark and Collip Method 50. Estimation of Reducing Sugar by Nelson-Somogyi Method 51. Estimation of Creatinine 52. Estimation of Urea by Dam-TSC Method 53. Estimation of Deoxyribonucleic Acid (DNA) by Diphenylamine Method 54. Estimation of Ribonucleic Acid (RNA) by Orcinol Method 55. Estimation of Formaldehyde by Sodium Hypoiodite Method 56. Estimation of Inorganic Phosphorus by Fiske-Subbarow Method 57. Enzyme Analysis 57.1. Effect of amylase activity on starch by Dinitro salycylic acid method 57.2. ATPase 57.3. Lipase 57.4. Trypsin 57.5. Estimation of Serum Acid Phosphatase activity 57.6. Estimation of Serum Alanine Transaminase 57.7. Estimation of Serum Alkaline Phosphatase 57.8. Estimation of Serum Aspartate Transaminase 57.9. Estimation of Serum Lactate Dehydrogenase 58. Restriction Digestion of DNA 59. DNA Ligation 60. Competent Cell Preparation and Transformation 61. Isolation of DNA from Tissue Sample 62. Polymerase Chain Reaction (PCR) 63. Recovery of DNA from Agarose Gels64. RNA Isolation 65. Ouchterlony Double Diffusion 66. Circular Paper Chromatography 67. Enzyme Linked Immunosorbent Assay (ELISA) 68. Western Blotting 69. RNA Extraction and RT-PCR 70. Preparation of Normal Solution of Acid and Base, Buffers and Reagents.…