Lipid Rafts: Methods and Protocols (Methods in Molecular Biology (2187))

ISBN 10: 1071608134 / ISBN 13: 9781071608135

Published by Humana Press, 2020

About this Item

Seller Inventory # 26377051472

Bibliographic Details

• Title: Lipid Rafts: Methods and Protocols (Methods in Molecular Biology (2187))
• Publisher: Humana Press
• Publication Date: 2020
• Language: English
• ISBN 10: 1071608134
• ISBN 13: 9781071608135
• Binding: Hardcover
• Condition: New

About this title

Synopsis

This volume gives a comprehensive insight into established and novel methods to analyze the structure and function of lipid rafts. This book covers topics such as isolation of lipid rafts and their functional analysis using biochemical methods; visualization of lipid rafts and their interaction with proteins using fluorescence-related methods; preparation of giant lipid vesicles and fluorescence spectroscopy; FRET and FRAP; and using photo-activated cross-linking of a ceramide analog combined with proximity ligation assay. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Lipid Rafts: Methods and Protocols is a valuable resource for both novice and expert researchers interested in learning moreabout the function of lipid rafts in many areas of cell biology and medicine.