Live-Cell RNA Imaging | Methods and Protocols

Shin-Ichi Sato

ISBN 10: 1071642502 / ISBN 13: 9781071642504

Published by Springer, 2025

About this Item

Live-Cell RNA Imaging | Methods and Protocols | Shin-Ichi Sato | Taschenbuch | Methods in Molecular Biology | xi | Englisch | 2025 | Springer | EAN 9781071642504 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu.

Seller Inventory # 136124888

Bibliographic Details

• Title: Live-Cell RNA Imaging | Methods and Protocols
• Publisher: Springer
• Publication Date: 2025
• Language: English
• ISBN 10: 1071642502
• ISBN 13: 9781071642504
• Binding: Taschenbuch
• Condition: Neu
• Dimensions: 254W x 178H x 13D millimeters 254 millimeters width by 178 millimeters height by 13 millimeters depth
• Weight: 424 grams
• Seller catalogs:
  • Bücher

About this title

Synopsis

This detailed volume presents a comprehensive guide to live-cell RNA imaging techniques and methodologies that have been successfully used to study the dynamics or function of various RNAs in cells. The topics covered encompass techniques including the detection of aptamer-tagged mRNA molecules utilizing fluorogenic proteins, monitoring endogenous mRNA via a split-protein-based probe within neuronal cells, sensitive detection of low-abundance RNAs employing catalytic hairpin assembly circuits, monitoring RNA dynamics using the MS2 system within plant cells, detection of miRNA utilizing DNA-MOF probes, and identification of RNA G-quadruplex structures or nucleolar RNA through fluorescent probes, among other advanced techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

 

Authoritative and practical, Live-Cell RNA Imaging: Methods and Protocols provides researchers with a diverse toolkit for investigating RNA dynamics and function within live cells, thereby contributing to advancements in our understanding of cellular processes at the molecular level.