Livestock Genome Editing Tools

• Provides operable experimental procedures of pigs, cattle, and sheep genome editing tools
• Introduces the evaluation, breeding process, and application of each of the latest and most effective tools
• Examines the importance of livestock germplasm innovation, breed improvement, and human disease model generation

Dr. Kui Li is a Professor at Chinese Academy of Agricultural Sciences. He graduated from Wuhan University of China and appointed as a full Professor at Huazhong (Central China) Agricultural University and Chinese Academy of Agricultural Sciences in China since 1996, respectively. He used to be a visiting scholar at the University of Sydney, Australia and a full-time visiting Professor at the Cornell University, USA. Prof. Li’s research interests include pig genome and breeding, pig genetic engineering, experimental mini-pigs and medical models. He has been working on these fields for more than 3 decades. Since 1996, Prof. Li systematically compared the multi-omics of different developmental stages of Chinese and Western pig breeds and found lots of multi-level molecular markers. He also identified several hundreds of molecular markers related to porcine economic traits which are used to develop pig breeds. He established steady platforms for porcine somatic cell nuclear transfer, pronuclear injection, and genome editing techniques. He also established the techniques to develop genetic modified pigs with multi-gene compound traits, controllable expression and friendly gene locus site directed integration. Excitingly, Prof. Li’s research group generated more than 20 new breeding materials for agriculture including MSTN-edited pigs, anti-porcine reproductive and respiratory syndrome pigs. His research group also generated genome edited pigs for biomedicine including cardiovascular disease and diabetes models, which showed great application perspective. Prof Li is a founder of Agsino Gensources Co., Ltd., an animal biotechnology breeding company.

Dr. Bingyuan Wang is an Associate Professor at the Institute of Animal Sciences, Chinese Academy of Agricultural Sciences. In 2014, she graduated from Max Planck Institute for Molecular Biomedicine in Germany. From 2015 to 2023, she worked in the Porcine Gene Engineering and Plasma Innovation group, which is led by Prof. Kui Li. Her research interests include the molecular mechanisms underlying porcine spermatogonial cells proliferation and apoptosis, the influence of exogenous factors in the porcine reproduction using porcine oocytes and spermatogonial cells, induction of gametes from porcine spermatogonial cells, and the generation of genome-edited pigs using porcine spermatogonial cells.