497 p. Contains: Illustrations. Audience: General/trade. A comprehensive source of technical guidance for experienced investigators and essential resource for newcomers to mammalian genetics and embryology. This edition (1st ed., 1986) is revised and expanded to incorporate improvements to existing methods as well as new technologies. It contains new sections on the production and analysis of transgenic mice, the manipulation of preimplantation embryos to generate chimeras, the culture and manipulation of embryonic stem cells, including gene "knockouts, " and techniques for visualizing genes, gene products, and specific cell types. As before, included with the protocols is a summary of current understanding of mouse development at a molecular level. Plastic comb-binding. Annotation copyright Book News, Inc. Portland, Or. Very good. No dust jacket. light shelfwear, previous owner name and smudge inside cover, else very good, no jacket, 2nd ed. HARDCOVER.

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Bibliographic Details

Title: Manipulating the Mouse Embryo: A Laboratory Manual
Publisher: Cold Spring Harbor Laboratory Press
Publication Date: 1994
Language: English
ISBN 10: 0879693843
ISBN 13: 9780879693848
Binding: Hard cover
Edition: 2nd ed.

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Synopsis

A comprehensive source of technical guidance for experienced investigators and essential resource for newcomers to mammalian genetics and embryology. This edition (1st ed., 1986) is revised and expanded to incorporate improvements to existing methods as well as new technologies. It contains new sections on the production and analysis of transgenic mice, the manipulation of preimplantation embryos to generate chimeras, the culture and manipulation of embryonic stem cells, including gene "knockouts," and techniques for visualizing genes, gene products, and specific cell types. As before, included with the protocols is a summary of current understanding of mouse development at a molecular level. Plastic comb-binding. Annotation copyright Book News, Inc. Portland, Or.

Review

"....essential reading for those engaged in production and analysis of transgenic mice..." -- Immunology Today

"It is more than a compilation of essential protocols, it is a well-balanced textbook encompassing both theoretical and practical..." -- Genetic Research

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