Title: Membrane Protein Purification and Crystallization: A Practical Guide 2ed
Publisher: Academic Press
Publication Date: 2003
Language: English
ISBN 10: 0123617766
ISBN 13: 9780123617767
Binding: Soft cover
Edition: 2nd Edition
Condition: Brand New

About this title

Synopsis

This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins.

Provides general guidelines and strategies for isolation and crystallization of membrane proteins
Gives detailed protocols that have wide application, and low specialized equipment needs
Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins
Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes
Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization
Presents a comprehensive guide to 2D-crystallization of membrane proteins

About the Author

Deceased: February 12, 2017

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