Method Development in Analytical HPLC

Method Development in Analytical HPLC presents the essential information for understanding the process of developing an HPLC method of analysis. It includes foundational information related to HPLC, as well as discussion of sample types, the properties of analytes and matrices in the samples, and sample preparation. The core of the book describes the best ways for approaching method development in various types of HPLC and the criteria for method optimization and validation.

This book provides clear guidance for adopting analytical methods from the literature and describes the development of original methods with selection of the suitable type of HPLC, of specific columns, mobile phase, and detection techniques with an emphasis on the use of mass spectrometry for detection, as well as optimization and validation of the chosen analytical method. The book includes useful details on method development for specific types of chromatography such as RP-HPLC, HILIC, ion exchange, size exclusion, and chiral.


Method Development in Analytical HPLC also includes information about green chemistry in analytical methods, computer assisted method development, and other key contemporary aspects of the subject.

• Offers a systematic and logical presentation of the foundational of analytical HPLC
• Goes in-depth on method development for specific types of chromatography such as RP-HPLC, HILIC, ion exchange, and size exclusion
• Includes methods with an emphasis on the use of mass spectrometry for detection

Dr. Serban C. Moldoveanu held several teaching positions at the University of Bucharest, Romania, until 1983, when he immigrated to the United States. There, he continued teaching at the university level for several years before focusing primarily on industrial research. He served as Senior Principal Scientist at R.J. Reynolds Tobacco Company and retired at the end of 2023. His research has centered on various aspects of chromatography, including method development for the analysis of natural products using GC/MS, HPLC, and LC/MS/MS. He has also investigated the pyrolysis of a variety of polymers and organic compounds. Dr. Moldoveanu has published more than 150 articles in peer-reviewed journals, 8 books with Elsevier, approximately 22 patents/patent applications, and several book chapters. He was honored with the Tobacco Science Research Conference Lifetime Achievement Award in 2012 and currently serves as an editor for three chemistry journals.

Victor David was a Professor and Head of the Department of Analytical Chemistry, University of Bucharest, Romania. Recently retired, he remains Emeritus Professor at the same Department. His main field of research is separation science (theory and applications in various scientific domains). He is the author of more than 200 publications, including over 150 scientific papers in peer reviewed journals and 25 books and chapters. He is member of editorial board of Biomedical Chromatography, Molecules, Journal of Essential Oil-Bearing Plants and Revue Roumaine de Chimie.