Method For Simultaneous Estimation Of Glucosamine And Boswellic Acids: Development and Validation of Analaytical method by HPTLC

Badmanaban Ramalingam

ISBN 10: 3330015284 / ISBN 13: 9783330015289

Published by LAP LAMBERT Academic Publishing, 2016

About this Item

88 pages. 8.66x5.91x0.20 inches. In Stock.

Seller Inventory # 3330015284

Bibliographic Details

• Title: Method For Simultaneous Estimation Of Glucosamine And Boswellic Acids: Development and Validation of Analaytical method by HPTLC
• Publisher: LAP LAMBERT Academic Publishing
• Publication Date: 2016
• Language: English
• ISBN 10: 3330015284
• ISBN 13: 9783330015289
• Binding: Paperback
• Condition: Brand New
• Weight: 0.18 kilograms

About this title

Synopsis

Objective: The objective is to develop and validate simple, accurate, sensitive and rapid , HPTLC method for simultaneous estimation of Glucosamine (GLU) and β-Boswellic acids (BAs) in their combined tablet dosage form. Experimental Work: In HPTLC method, chromatographic separation was carried out on TLC plate pre-coated with silica gel 60F254 using mobile phase-A,Toluene-Ethylacetate (2.5:1.5 v/v) for 11-KBA and A-11-KBA,scanning the plate at 260 nm [scan-1] and mobile phase-B, 1-Butanol -Water-Glacial acetic acid (3:1:1, v/v/v) for GLU ,derivatized the plate by Ninhydrin reagent and scanning the plate at 485 nm[scan-2]. Results: The HPTLC method was found to be linear over the range of 6250-31250 , 300-1500 and 150-750 ng/band for GLU,11-KBA and A-11-KBA , respectively.In this method, % RSD of assay determination of both drugs was found to be less than 2.0 and mean % recoveries were found between 98-101 %. Conclusion: The method was found to be precise as % RSD of assay in all cases was less than 2.0. High percentage recoveries obtained in this method indicate that the method is accurate. There was no interference from any excipients in the determination of drugs in tablets.

About the Author

Working as Professor in Nirmala College of Pharmacy,Muvattupuzha,Kerala.Having 14 years of Teaching and Research Experience.Bagged AICTE Research Promotion Scheme Award of RS. 14.5 Lakhs.Since 2013 Approved as PhD Guide from JJTU University Rajasthan,India.Published more than 40 Research Publications in Reputed Journals.Guided 20 M.Pharm projects.