Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance energy transfer (FRET), lifetime imaging (FLIM), spectral imaging, fluorescence recovery after photobleaching (FRAP), optical tweezers, total internal reflection, high spatial resolution atomic force microscopy (AFM), and bioluminescence imaging for gene expression. The usage of these techniques in various biological applications, including calcium, pH, membrane potential, mitochondrial signaling, protein-protein interactions under various physiological conditions, and deep tissue imaging, is clearly presented. The authors describe the approaches to selecting epifluorescence microscopy, the detectors, and the image acquisition and processing software for different biological applications. Step-by-step directions on preparing different digital formats for light microscopy images on websites are also provided.

About the Author

Ammasi Periasamy, Professor Biology and Biomedical Engineering, University of Virginia.

"About this title" may belong to another edition of this title.

Store Description

WELCOME TO MY BOOKSTORE! I OFFER A CAREFULLY SELECTED COLLECTION OF USED, VINTAGE, AND HARD-TO-FIND BOOKS ACROSS A VARIETY OF GENRES. EVERY ITEM IS DESCRIBED HONESTLY AND SHIPPED PROMPTLY WITH CARE. FAST,SECURE SHIPPING-TYPICALLY WITHIN 1 BUSINESS DAY ACURATE DESCRIPTIONS-I AIM FOR TRANSPARENCY IN EVERY LISTING ...? QUESTIONS? I RESPOND QUICKLY AND POLITELY I VALUE EACH CUSTOMER AND STRIVE TO MAKE EVERY PURCHASE SMOOTH AND SATISFYING.THANK YOU FOR SUPPORTING A SMALL, INDEPENDENT BOOKSELLER!

Visit Seller's Storefront

Seller's business information

YESIBOOKSTORE
FL, U.S.A.

Sale & Shipping Terms

Terms of Sale

Shipping Terms

Shipping rates within U.S.A.

Shipping rates within U.S.A.
Order quantity	6 to 19 business days	4 to 12 business days
First item	US$ 0.00	US$ 25.00

Delivery times are set by sellers and vary by carrier and location. Orders passing through Customs may face delays and buyers are responsible for any associated duties or fees. Sellers may contact you regarding additional charges to cover any increased costs to ship your items.