Methods in Enzymology: Bacterial Pathogenesis: Identification and Regulation of Virulence Factors (Volume 235.A)

This volume of Methods in Enzymology presents methods for the isolation and identification of bacterial pathogens and associated virulence determinants. It will enable the researcher to determine if closed genes or isolated gene products are involved in virulence.

• Animal model system to determine bacterial virulence
• Epidemiological techniques for the identification of bacterial strains and species
• Protocols for the purification of subcellular bacterial products frequently associated with virulence
• Determination of the means by which bacteria acquire iron
• Methods to generate mutants, to construct isogenic strains by allelic exchange, to identify bacterial genes only expressed during infection of the host, and to study regulation of selected bacterial virulence factors
• Methods for the assay of destructive bacterial enzymes and toxins

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