Methods in Enzymology Volume 182: Guide to Protein Purification

The Guide to Protein Purification is a self-contained volume covering all the important procedures for purifying proteins, as well as other more specialized techniques. However, to stay within the confines of a single volume, some details are dealt with by reference to other works, but these have been kept to a minimum. It is hoped that this volume will satisfy the needs of both the novice in protein purification and the more experienced researcher.

Key Features
* Contains procedures newly written for this volume
* Provides extensive practical information
* Clearly describes rationale and strategies for protein and enzyme purification
* Offers personal perspectives on enzyme purification by eminent researchers

Guide to Protein Purification, designed to serve the needs of the student, experienced researcher, and newcomer to the field is a comprehensive manual that presents in one source, all up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes.
Key features of the guide:
* Detailed procedures newly written for this volume
* Extensive practical information
* Rationale and strategies for protein and enzyme purification
* Personal perspectives on enzyme purification by eminent researchers
Among the topics covered:
* General methods for handling proteins and enzymes
* Extraction, subcellular fractionation, and solubilization procedures
* Comprehensive purification techniques
* Specialized purification procedures
* Protein characterization
* Immunological procedures
* Computer analysis of protein structure