Methods for Fragments Screening Using Surface Plasmon Resonance

Language: English

Published by Springer Nature Singapore Sep 2021, 2021

9811615357 / 9789811615351

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Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, GermanyBuchWeltWeit Ludwig Meier e.K.

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This item is printed on demand - it takes 3-4 days longer - Neuware -This volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR). The initial chapter of the book discusses the principle of SPR for the identification of biomolecular interactions, while the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-streptavidin coupling and methods to optimize the interactions. Next, it describes fragment preparation for screening in SPR and presents methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE). It reviews techniques of next-generation injections that improve the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. Finally, the book elucidates a comprehensive yet representative description of challenges associated with the molecular interaction of proteins using SPR. 132 pp. Englisch.…

Seller Inventory # 9789811615351

Title
Methods for Fragments Screening Using Surface Plasmon Resonance
Author
Ramachandraiah Gosu
Publisher
Springer Nature Singapore Sep 2021
Publication year
2021
Condition
Neu
Binding
Buch
Language
English
ISBN 10
9811615357
ISBN 13
9789811615351
Item weight
371 grams
Dimensions
241x160x13 mm

BuchWeltWeit Ludwig Meier e.K.

Bergisch Gladbach, Germany

5-star seller

AbeBooks seller since January 11, 2012

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BuchWeltWeit Ludwig Meier e.K.

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