Methods for Fragments Screening Using Surface Plasmon Resonance

Language: English

Published by Springer Nature Singapore, Springer Nature Singapore Sep 2022, 2022

9811615381 / 9789811615382

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This item is printed on demand - Print on Demand Titel. Neuware -This volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR). The initial chapter of the book discusses the principle of SPR for the identification of biomolecular interactions, while the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-streptavidin coupling and methods to optimize the interactions. Next, it describes fragment preparation for screening in SPR and presents methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE). It reviews techniques of next-generation injections that improve the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. Finally, the book elucidates a comprehensive yet representative description of challenges associated with the molecular interactionof proteins using SPR.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 132 pp. Englisch.…

Seller Inventory # 9789811615382

Title
Methods for Fragments Screening Using Surface Plasmon Resonance
Author
Ramachandraiah Gosu
Publisher
Springer Nature Singapore, Springer Nature Singapore Sep 2022
Publication year
2022
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
9811615381
ISBN 13
9789811615382
Item weight
213 grams
Dimensions
235x155x8 mm

buchversandmimpf2000

Emtmannsberg, BAYE, Germany

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