Methods in Practical Laboratory Bacteriology

Henrik Chart, Elwyn Griffiths, Mary E. Kaufmann, David G. Pitcher, Tyrone L. Pitt, Andrea Thomas, Neil Woodford, Jennifer A. Frost, Pauline Stevenson, Alan P. Johnson, E. John Threlfall

ISBN 10: 1138452009 / ISBN 13: 9781138452008

Published by CRC Press 2018-06-08, 2018

About this Item

Seller Inventory # 6666-TNFPD-9781138452008

Bibliographic Details

• Title: Methods in Practical Laboratory Bacteriology
• Publisher: CRC Press 2018-06-08
• Publication Date: 2018
• Language: English
• ISBN 10: 1138452009
• ISBN 13: 9781138452008
• Binding: Hardcover
• Condition: New

About this title

Synopsis

The success of laboratory experiments relies heavily on the technical ability of the bench scientist, with the aid of "tricks-of-the-trade", to generate consistent and reliable data. Regrettably, however, these invaluable "tricks-of-the-trade" are frequently omitted from scientific publications. This paucity of practical information relating to the conduct of laboratory bacteriology experiments creates a gaping void in the pertinent literature. Methods in Practical Laboratory Bacteriology fills this void. It provides detailed technical information that ensures that you achieve consistent and reliable data. The book addresses the aspects of bacterial fractionation and membrane characterization, the analysis of Lipopolysaccharides and the techniques of SDS-PAGE, immunoblotting, and ELISA. It also describes the methods used for detecting and quantifying bacterial resistance to antibiotics, and the analysis of bacterial chromosomes by pulsed-field gel electrophoresis (PFGE). Methods in Practical Laboratory Bacteriology also covers protocols for extracting the fingerprinting plasmids, as well as the use of non-radio labeled gene probes and ribosomal RNA gene probes.

About the Author

Henrik Chart