Title: Methods for Transfecting Cells with Nucleic Acids of Animal Viruses: a Review (Experientia Supplementum, 16) (German Edition)
Publisher: Birkh�user
Publication Date: 2014
Language: German
ISBN 10: 3034857748
ISBN 13: 9783034857741
Binding: Soft cover
Condition: New

About this title

Synopsis

Transfection, which is the infection of a cell with naked viral nucleic acid with the consequent production of complete virus, was first reported in 1956 for the ribonucleic acid of tobacco mosaic virus. Many reports of transfection, and several reviews of the field, have appeared since then. Crucial for the demonstration of transfection is that the viral nucleic acid is not damaged in the process of obtaining it from the virions, or from the infected tissue. To this end, procedures are designed to minimize the possibili- ties of degradation of the viral nucleic acid by nucleases present in the biolo- gical source. The most common method for preparing viral nucleic acid is the phenol method in which virus or infected-tissue preparations are extracted with phenol. Much of the protein goes down into the phenol phase, whereas the viral nucleic acid stays up in the aqueous phase. The transfection methods for animal virus nucleic acids are of three major kinds: (a) hypertonic methods; (b) insoluble facilitator methods; and (c) polycation methods. These methods have wide applicability to vertebrate celli animal virus NA systems, but anyone method does not seem to be highly effective for all such systems with the possible exception of the method using the polycation diethylaminoethyl-dextran. The insoluble facilitator method shows astonishing 'cell-specificity'; that is, it is a very effective method for transfecting some kinds of vertebrate cells, but nearly ineffective for some other kinds.

"About this title" may belong to another edition of this title.

Store Description

Hello! Ria Christie Collections is an online venture that was initially set up in 2012 to sell books. We do not have a physical high street store. We are professional online booksellers. We only sell brand new books in perfect condition that we source from various suppliers and the publishers. Primarily, our aim is to provide an excellent service to all our customers. We always work as a team to achieve this. Our other objectives are to: 1. Ensure that all our products reach their destination quickly in a safe and secure manner 2. Answer to all our customer queries within 24 hours 3. Ensure that our customers are happy with their purchases 4. Provide all the items at a competitive price 5. Always listen to our customers Ria Christie Collections is not a registered company. It is a Sole Trader venture. Other key information is shown below: Contact Person Name: Rakesh Luchmun (Mr) Storefront Name: Ria Christie Collections Place of Establishment Address: Suite B; ARUN House; ARUN Building Arundel Road Uxbridge UB8 2RR United Kingdom E-Mail Address: riachristie@hotmail.co.uk VAT Number: GB 160 5650 25 We always work hard and aim to comply with all of Abebooks Policies. If you have any issues, please do not hesitate to write to us whether before or after a purchase. We promise to reply to you promptly and, in any case, within 24 hours. Thank you kindly! Yours sincerely Mr Rakesh Luchmun (Founder) and the Ria Christie Collections Team

Visit Seller's Storefront

Seller's business information

Ryefield Investments Limited
175 Pield Heath Road, Uxbridge, UB8 3NL, United Kingdom

Sale & Shipping Terms

Terms of Sale

All Returns and Refund are as per Abebooks policies.

Shipping Terms

Orders usually ship within 2 business days. If your book order is heavy or oversized, we may contact you to let you know extra shipping is required. Thank you!