Methods in Yeast Genetics 2005: A Cold Spring Harbor Laboratory Course Manual

"Methods in Yeast Genetics" is a course that has been offered annually at Cold Spring Harbor Laboratory for the last thirty years. This is an updated edition of the course manual, which provides a set of teaching experiments, along with protocols and recipes for the standard techniques and reagents used in the study of yeast biology. Since the last edition of the manual was published (2000), revolutionary advances in genomics and proteomics technologies have had a significant impact on the field. This updated edition reflects these advances, and also includes new techniques involving vital staining, visualization of Green Fluorescent Protein, new drug resistance markers, high-copy suppression, Tandem Affinity Protein tag protein purification, gene disruption by double-fusion polymerase chain reaction, and many other recent developments.

This update of the 2000 edition is the latest version of a book that remains the best single source of basic genetics techniques for Saccharomyces cerevisiae... Based on the long running course at Cold Spring Harbor, it contains a mixture of experiments, techniques, protocols and basic data. The experiments include very clear explanations of the logic behind the approaches. New techniques like TAP tagging are included and many familiar experiments have been revised to take into account, for example, cytological advances with better fluorophores and GFP-fusions, and the ready availability of systematic deletion strains. It is an essential purchase for all laboratories. Microbiology Today --Cold Spring Harbor