Microarray Analysis : Biochips and Eradication of all Diseases

Kal Sharma

ISBN 10: 1606506676 / ISBN 13: 9781606506677

Published by Momentum Press, 2015

About this Item

nach der Bestellung gedruckt Neuware - Printed after ordering - Microarray Analysis is a natural outgrowth of the author's teaching and research into the field of microarray processing, a fast growing and developing field. There has been a reduction in the genominomics of sequencing of DNA and proteins: from $3 billion in HGP costs down to $1000 per genome in 2014 using next-generation sequencers.Different sequencing methods in place and various applications of these methods are illustrated. Commercially available Next-Generation Sequencers are compared side-side by side. This book will show how the biochemical reactions involved in the different steps of the cure process can be studied using microarray methods. Examples will be provided throughout the text of various disease states, including the spread of cystic fibrosis in children, consanguineous marriages in Saudi Arabia, genetic disorders, and autoimmune disorders. The protocols from sample preparation to confocal scanning microscopy and detectors using photo multiplier tubes to statistical data analysis methods will be discussed in detail. The Polymerase chain reaction, a technique used to amplify and simultaneously quantify a targeted DNA molecule is explored in relation to the best surface to be used for Microarray Analysis. Sanger sequencing, sequencing by synthesis, sequencing by ligation, single molecule sensing, DNA passage through nanopores in graphene are some methods used for obtaining sequence distribution information.

Seller Inventory # 9781606506677

Bibliographic Details

• Title: Microarray Analysis : Biochips and Eradication of all Diseases
• Publisher: Momentum Press
• Publication Date: 2015
• Language: English
• ISBN 10: 1606506676
• ISBN 13: 9781606506677
• Binding: Taschenbuch
• Condition: Neu
• Dimensions: 229W x 152H x 18D millimeters 229 millimeters width by 152 millimeters height by 18 millimeters depth
• Weight: 477 grams

About this title

Synopsis

Microarray Analysis will be developed as a natural outgrowth of the authors teaching and research into the field of Microarray processing, a fast growing and developing field. The book will discuss the development of various microarray analysis tools and the applications of the technology towards disease prevention and eradication. The goal of the book is to illustrate the many sequencing methods in place, and to show the various applications of these methods. This book will show how the biochemical reactions involved in the different steps of the cure process can be studied using microarray methods. Examples will be provided throughout the text of various disease states, including the spread of cystic fibrosis in children from consanguineous marriages in Saudi Arabia, genetic disorders and, and autoimmune disorders. The protocols from sample preparation to confocal scanning microscopy and detectors using photo multiplier tubes will be discussed in detail. The Polymerase chain reaction, a technique used to amplify and simultaneously quantify a targeted DNA molecule will be explored in detail in relation to the best surface to use for Microarray Analysis. Additionally, Microarray Analysis: Biochips and Eradication of Disease will feature the statistical methods needed for analysis of DNA data from microarray analysis, including shotgun and next generation sequencing. The applications of these methods including data normalization, cluster analysis and predictions from a microarray data set, will be examined in detail. Finally, the statistical design of experiments, computing methods for linear and nonlinear regression, robust estimation and general maximum-likelihood methods will be explained for use in future experiments.

About the Author

Houston, TX; Texas Southern University