Microbial Gene Essentiality: Protocols and Bioinformatics (Methods in Molecular Biology)

Andrei L. Osterman (Editor), Svetlana Y. Gerdes (Editor)

ISBN 10: 1588293785 / ISBN 13: 9781588293787

Published by Humana Press, 2008

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Bibliographic Details

• Title: Microbial Gene Essentiality: Protocols and Bioinformatics (Methods in Molecular Biology)
• Publisher: Humana Press
• Publication Date: 2008
• Language: English
• ISBN 10: 1588293785
• ISBN 13: 9781588293787
• Binding: Hardcover
• Condition: New
• Seller catalogs:
  • LIFE SCIENCES

About this title

Synopsis

Microbial Gene Essentiality: Protocols and Bioinformatics contains a comprehensive collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies, developed and presented by the leading groups in the field. In addition to wet-lab protocols, the book describes (i) statistical methods essential for planning successful large-scale essentiality screens, as well for data evaluation and analysis; (ii) in-silico prediction of gene essentiality using genome-scale reconstructed metabolic models; and (iii) data integration and comparative analysis in the context of genomic databases. This volume provides researchers with a first-stop guide for choosing the most appropriate strategy for planned essentiality studies. Experimental and computational aspects are equally important in genome-scale gene essentiality analysis, as in all other genomic technologies, and Microbial Gene Essentiality: Protocols and Bioinformatics reflects both of these aspects. All protocols follow the successful Methods in Molecular Biology™ series format, each offering an introduction outlining the principles behind the techniques, step-by-step instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls, and are intended for both novice and expert scientists.