The Molecular Biology of Bacterial Virus Systems

Language: English

Published by Springer, 2011

3642731171 / 9783642731174

Series: Book 301 of 322 - Current Topics in Microbiology and Immunology

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Druck auf Anfrage Neuware - Printed after ordering - The fact that none of the known DNA polymerases is able to initiate DNA chains but only to elongate from a free 3' -OH group raises the problem of how replication is initiated, both at the replication origin and on Okazaki frag ments. It was first shown by A. KORNBERG et al. that a general mechanism to initiate replication is through the formation of an RNA primer catalyzed by RNA polymerases or by a new class of enzymes, the primases (KORNBERG 1980). This mechanism, which can be used in the case of circular DNA molecules or linear DNAs that circularize or form concatemers, cannot be used at the ends of linear DNAs since the RNA primer is removed from the DNA chain, and there is no way of filling the gap resulting at the 5' -ends of the newly synthesized DNA chain. In some cases linear DNA molecules contain a palin dromic nucleotide sequence at the 3' -end that allows the formation of a hairpin structure which provides the needed free 3'-OH group for elongation. This mechanism, first proposed by CAVALIER-SMITH (1974) for eukaryotic DNA repli cation, was shown to take place in several systems (KORNBERG 1980, 1982). Another mechanism to initiate replication consists in the specific nicking of one of the strands of a circular double-stranded DNA, producing a 3'-OH group available for elongation (KORNBERG 1980).

Seller Inventory # 9783642731174

Title
The Molecular Biology of Bacterial Virus Systems
Author
Rudolf Rott
Publisher
Springer
Publication year
2011
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
3642731171
ISBN 13
9783642731174
Item weight
194 grams
Dimensions
242x170x7 mm
Series
Book 301 of 322: Current Topics in Microbiology and Immunology

AHA-BUCH GmbH

Einbeck, Germany

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AbeBooks seller since August 14, 2006

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