Molecular Biology Problem Solver : A Laboratory Guide

Alan S Gerstein

ISBN 10: 0471379727 / ISBN 13: 9780471379720

Published by Wiley Okt 2001, 2001

About this Item

Neuware - Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as:\* Have you selected the most appropriate research strategy \* Have you identified the issues critical to your successful application of a technique \* Are you familiar with the limitations of a given technique \* When should common procedural rules of thumb not be applied \* What strategies could you apply to resolve a problem A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include:\* Working safely with biological samples and radioactive materials\* DNA and RNA purification\* PCR\* Protein and nucleid acid hybridization\* Prokaryotic and eukaryotic expression systems\* Properly using and maintaining laboratory equipment; Der 'Molecular Biology Problem Solver' ist ein einzigartiger Laborleitfaden für Molekularbiologen. Hier lernen Sie, die kritischen Elemente von Techniken, Reagenzien und Verfahren zu erkennen und Ihre Fähigkeiten zur Fehleranalyse und -behebung bei der Durchführung von Experimenten deutlich zu verbessern. Der hier behandelte Stoff dient als Grundlage für die Entwicklung fundierter analytischer Laborkenntnisse. Damit bietet dieses Buch eine solide Basis für ein besseres Verständnis möglicher Fehlerursachen im Laboralltag. Es soll der Forschungsgemeinschaft - vom Wissenschaftler bis hin zum Techniker - dabei helfen, unabhängig verschiedene Situationen und Problemlösungen auszuarbeiten und künftig Probleme zu vermeiden. Schwerpunktmäßig werden u.a. folgende Themen behandelt: Gefährliche Stoffe, Benutzung und Wartung der Laboreinrichtung, Bakteriologie, DNA und RNA und Elektrophorese. Der 'Molecular Biology Problem Solver' liefert eine ausgewogene Darstellung von Theorie und Praxis und enthält wichtige Informationen, die anderenorts schwer zugänglich sind und die auch in den sehr umfangreichen und detaillierten Current Protocols Series nicht aufgeführt sind. Damit wird dieses Buch zu einer unverzichtbaren Informationsquelle und zu einem absoluten Muss für jeden Molekularbiologen.

Seller Inventory # 9780471379720

Bibliographic Details

• Title: Molecular Biology Problem Solver : A Laboratory Guide
• Publisher: Wiley Okt 2001
• Publication Date: 2001
• Language: English
• ISBN 10: 0471379727
• ISBN 13: 9780471379720
• Binding: Taschenbuch
• Condition: Neu
• Dimensions: 254W x 178H x 31D millimeters 254 millimeters width by 178 millimeters height by 31 millimeters depth
• Weight: 1094 grams

About this title

Synopsis

Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems.

Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as:

• Have you selected the most appropriate research strategy?
• Have you identified the issues critical to your successful application of a technique?
• Are you familiar with the limitations of a given technique?
• When should common procedural rules of thumb not be applied?
• What strategies could you apply to resolve a problem?

A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include:

• Working safely with biological samples and radioactive materials
• DNA and RNA purification
• PCR
• Protein and nucleid acid hybridization
• Prokaryotic and eukaryotic expression systems
• Properly using and maintaining laboratory equipment

About the Author

ALAN GERSTEIN and his collaborators have been guiding researchers of all levels of expertise through the troubleshooting process over the course of three decades, assuring that Molecular Biology Problem Solver will prove an excellent resource for all life scientists.