Muscle Stem Cells: Methods and Protocols (Methods in Molecular Biology, 3093)

This detailed volume provides an experimental roadmap for interrogating muscle stem cell biology, from tissue dissociation and cell isolation to ex vivo analysis, in vitro modeling, functional assays, and multi-omics technologies. The book explores how to capture or preserve the physiological state of muscle stem cells in situ and ex vivo, techniques for in situ imaging, computational analysis of immunofluorescence, cross-grafting, and co-culture with niche-associated cells, as well as protocols applicable to human muscle stem cells and skeletal muscle tissue and approaches to generate human muscle progenitors and model human muscle stem cell fate in a dish. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, Muscle Stem Cells: Methods and Protocols serves as a useful resource for both new and experienced investigators working in the field of muscle stem cell biology in order to facilitate rigorous experimentation, methodological innovation, and further discovery in skeletal muscle regeneration.

This detailed volume provides an experimental roadmap for interrogating muscle stem cell biology, from tissue dissociation and cell isolation to ex vivo analysis, in vitro modeling, functional assays, and multi-omics technologies. The book explores how to capture or preserve the physiological state of muscle stem cells in situ and ex vivo, techniques for in situ imaging, computational analysis of immunofluorescence, cross-grafting, and co-culture with niche-associated cells, as well as protocols applicable to human muscle stem cells and skeletal muscle tissue and approaches to generate human muscle progenitors and model human muscle stem cell fate in a dish. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, Muscle Stem Cells: Methods and Protocols serves as a useful resource for both new and experienced investigators working in the field of muscle stem cell biology in order to facilitate rigorous experimentation, methodological innovation, and further discovery in skeletal muscle regeneration.