Mycobacteria Protocols - Methods in Molecular Biology Vol 101

Parish, Tanya ; Stoker, Neil G.

ISBN 10: 0896034712 / ISBN 13: 9780896034716

Published by Humana Press Inc., Totowa, New Jersey, U.S.A., 1998

About this Item

472 pages. Maroon cover with white text, and black and white inlays. General shelf wear to spine, corners and edges. Top page block is dusty. Outer page block has light fingerprint smudges. Text is clean, bright and tight throughout.

Seller Inventory # 142250

Bibliographic Details

• Title: Mycobacteria Protocols - Methods in Molecular Biology Vol 101
• Publisher: Humana Press Inc., Totowa, New Jersey, U.S.A.
• Publication Date: 1998
• Language: English
• ISBN 10: 0896034712
• ISBN 13: 9780896034716
• Binding: hardcover
• Condition: Very Good
• Dust Jacket Condition: No Dust Jacket
• Seller catalogs:
  • Health & Medicine

About this title

Synopsis

Laboratory experts present cutting-edge molecular biology techniques for studying the mycobacteria, a medically significant group of organisms responsible for both human and animal disease, including tuberculosis and leprosy. The methods range from basic culture techniques, cell fractionation, and nucleic acid purification to more advanced protocols for the introduction of cloned DNA into mycobacteria, the use of reporter genes, and the expression of foreign genes. There are also important techniques for mycobacteriophages, genome analysis, chemical and transposon mutagenesis, gene replacement, and analysis of gene expression, as well as such clinically useful techniques as antibiotic susceptibility testing, speciation, and RFLP analysis.

From the Back Cover

Due to the rising threat of tuberculosis and other mycobacterial infections, methods to study the biology of the mycobacteria and to improve diagnostic, therapeutic and preventative reagents are still very much in need.  Mycobacteria Protocols, Second Edition updates and refines the methods of the well-received first edition while adding newly developed methods culled from the most cutting-edge research in the field.  Ranging from the basics of sub-cellular fractionation to advanced methods using specialized growth conditions and whole genome, transcriptome and proteome analysis, the chapters conform to the Methods in Molecular Biology™ series format, providing step-by-step laboratory protocols, lists of necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, Mycobacteria Protocols, Second Edition is the perfect resource to promote and stimulate further research into these intriguing, important and most fractious of bacteria.