Myogenesis and Skeletal Muscle Biology: Methods and Protocols (Methods in Molecular Biology, 3057)

This volume offers an extensive and forward-looking toolkit for the study of myogenesis and skeletal muscle biology, spanning stem cell isolation, tissue engineering, advanced imaging, and functional analysis. The book covers state-of-the-art methods for the isolation, expansion, and differentiation of skeletal muscle satellite cells and human induced pluripotent stem cells, including innovative approaches for generating 3D contractile and vascularized skeletal muscle tissue. A strong emphasis is placed on cutting-edge analytical techniques, with protocols describing ultrastructural fiber and sarcomeric imaging, high resolution fiber type microscopy, flow cytometry-based quantification of differentiation and reactive oxygen species, and advanced microscopy approaches ranging from light-sheet fluorescence imaging to focused ion beam scanning electron microscopy, among other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, Myogenesis and Skeletal Muscle Biology: Methods and Protocols serves as an indispensable resource for both trainees entering the field and experienced researchers seeking reliable and reproducible methodologies.

Chapter 7 is available open access under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License via link.springer.com.

This volume offers an extensive and forward-looking toolkit for the study of myogenesis and skeletal muscle biology, spanning stem cell isolation, tissue engineering, advanced imaging, and functional analysis. The book covers state-of-the-art methods for the isolation, expansion, and differentiation of skeletal muscle satellite cells and human induced pluripotent stem cells, including innovative approaches for generating 3D contractile and vascularized skeletal muscle tissue. A strong emphasis is placed on cutting-edge analytical techniques, with protocols describing ultrastructural fiber and sarcomeric imaging, high resolution fiber type microscopy, flow cytometry-based quantification of differentiation and reactive oxygen species, and advanced microscopy approaches ranging from light-sheet fluorescence imaging to focused ion beam scanning electron microscopy, among other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, Myogenesis and Skeletal Muscle Biology: Methods and Protocols serves as an indispensable resource for both trainees entering the field and experienced researchers seeking reliable and reproducible methodologies.

Chapter 7 is available open access under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License via link.springer.com.