Neutrophil Methods and Protocols provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Parts 1 & 2 provide an overview of neutrophils the methods most commonly used for isolating them from humans and other animal species. Part 3 details methods for investigating adhesion, chemotaxis, phagocytosis and bacterial activity. Part 4 presents protocols measuring gene expression in neutrophils, and Part 5 concludes with assays for diagnosing the most common neutrophil disorders. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.

Review

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"...a carefully compiled collection of protocols that allow for study of many aspects of the neutrophil." -Doody's Book Review, Weighted Numerical Score:85-3 Stars

"This excellent book is one of the latest in the ‘Methods in Molecular Biology’ series ... . it comes beautifully presented and bound in a hardback cover and would grace any bookshelf. I would urge all self-respecting laboratories working with neutrophils – whether in research or diagnostics – to purchase one immediately. ... I was impressed by this book’s comprehensive provision of protocols, addressing all aspects of neutrophil function and physiology, and including an excellent section on diagnosis of both inherited and non-inherited neutrophil disorders." (Lynn Morgan, Immunology News, May, 2008)

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