Neutrophil Methods and Protocols, Second Edition (Volume 1124)
Language: English
Published by Springer, 2019
- Hardcover
- New
Seller: Salish Sea Books, Nanaimo, BC, CanadaSalish Sea Books
AbeBooks seller since September 23, 2026
Condition: New
US$ 49.95
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This expansive volume serves as a critical laboratory resource focusing entirely on neutrophils, which function as the human body's frontline cellular defense mechanism against infectious diseases. The book provides a thorough overview of the role these crucial white blood cells play in host defense systems and overall inflammatory responses. Readers will find meticulously detailed procedures covering the isolation of neutrophils from both human subjects and various animal models. Furthermore, the text delves deeply into advanced cellular techniques, offering guidance on the subcellular fractionation of human neutrophils and the complex analysis of in vivo transmigrated cells. It also explores cutting-edge laboratory practices, including the generation of fully mature neutrophils utilizing induced pluripotent stem cells (iPSCs) and the comprehensive analysis of specific neutrophil gene expressions. In addition to isolation and generation techniques, the volume thoroughly outlines practical methods for evaluating functional cellular responses. This includes detailed experimental designs for investigating cell priming, the production of oxidants, phagocytosis processes, overall bactericidal activity, and the intricate formation of neutrophil extracellular traps (NETs). Researchers investigating cellular movement and communication will also benefit from dedicated protocols covering neutrophil adhesion mechanisms, chemotaxis, and the pathways involved in outside-in integrin signaling. Adhering to the highly acclaimed and user-friendly format of the Methods in Molecular Biology series, every individual chapter is engineered for immediate laboratory application. Chapters consistently feature introductory overviews of the specific topic, complete and exhaustive lists of all required laboratory materials and chemical reagents, and readily reproducible, step-by-step procedural guidelines. Crucially, each section concludes with expert notes on troubleshooting, offering invaluable advice on how to bypass common experimental errors and known pitfalls. Ultimately, this authoritative and highly accessible text stands as an indispensable tool for both basic scientists and clinical researchers seeking to apply the most modern methodological advances in their ongoing work with neutrophil biology.…
Seller Inventory # 4
- Title
- Neutrophil Methods and Protocols, Second Edition (Volume 1124)
- Author
- Mark T. Quinn, Frank R. DeLeo
- Publisher
- Springer
- Publication year
- 2019
- Condition
- New
- Binding
- Hardcover
- Language
- English
- ISBN 10
- 1627038442
- ISBN 13
- 9781627038447
- Edition
- 2nd Edition
- Item weight
- 1.5 kilograms
Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. Neutrophil Methods and Protocols, Second Edition provides a concise set of protocols written by leading researchers in the field for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step diagnostic assays of common neutrophil disorders. Topics covered include an overview of neutrophils and their role in host defense and inflammation; methods most commonly used for isolating neutrophils from humans and other animal species; procedures for subcellular fractionation of human neutrophils, analysis of in vivo transmigrated neutrophils, generation of mature neutrophils from induced pluripotent stem cells and analysis of neutrophil gene expression; methods for investigating priming, oxidant production, phagocytosis, bactericidal activity and extracellular trap formation and protocols for investigating neutrophil adhesion, chemotaxis and outside-in signaling via integrins. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Neutrophil Methods and Protocols, Second Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.
"Synopsis" may belong to another edition of this title.
From the Back Cover
Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. Neutrophil Methods and Protocols, Second Edition provides a concise set of protocols written by leading researchers in the field for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step diagnostic assays of common neutrophil disorders. Topics covered include an overview of neutrophils and their role in host defense and inflammation; methods most commonly used for isolating neutrophils from humans and other animal species; procedures for subcellular fractionation of human neutrophils, analysis of in vivo transmigrated neutrophils, generation of mature neutrophils from induced pluripotent stem cells, and analysis of neutrophil gene expression; methods for investigating priming, oxidant production, phagocytosis, bactericidal activity, and extracellular trap formation; and protocols for investigating neutrophil adhesion, chemotaxis, and outside-in signaling via integrins. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Neutrophil Methods and Protocols, Second Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.
"About the title" may belong to another edition of this title.
Salish Sea Books
Nanaimo, BC, Canada
AbeBooks seller since September 23, 2026
Shipping rates from Canada to U.S.A.
| Item | 7 to 14 business days | 5 to 8 business days |
|---|---|---|
| First item | US$ 12.99 | US$ 37.00 |
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Salish Sea Books is located in Nanaimo, British Columbia, specializing in a diverse, curated selection of used books and media. As a senior, one-man show, I handle every single aspect of this business personally. Because of this, I care deeply and passionately about getting your order right and providing genuine care for my customers. My ultimate goal is to ensure you know exactly what you are buying before you click purchase, and that you receive the highest level of personal service from my shelf to your door.…
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Salish Sea Books
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