Nitric Oxide Part B: Physiological and Pathological Processes (Methods in Enzymology)

Nitric oxide, recently named"Molecule of the Year"by the journal Science, impinges on a wide range of fields in biological research, particularly in the areas of biomedicine and cell and organismal biology, as well as in fundamental chemistry. This volume and its companion Volume 268 highlight methods used to research the physiology and toxicity of nitric oxide in cellular and organismal systems. It will be a valuable resource for the experienced researcher as well as for those newly entering the field.

• Effects of nitric oxide in cells and tissues
• Biochemical reactivity
• Tissue-specific effects
• Endothelial nitric oxide synthase expressions in heterologous systems
• Cellular signaling
• Pathological and clinical aspects of NO
• Fingerprints of pathological action
• Interaction with membranes, proteins, and nucleic acids
• Peroxynitrite
• Tissue and cellular cytotoxicity
• Clinical aspects

Helmut Sies, MD, PhD (hon), studied medicine at the universities of Tübingen, Munich, and Paris. He was the professor and chair of the Institute for Biochemistry and Molecular Biology I at Heinrich-Heine-University Düsseldorf, Germany, where he is now professor emeritus. He is a member of the German National Academy of Sciences Leopoldina and was the president of the North Rhine-Westphalian Academy of Sciences and Arts. He was named ‘Redox Pioneer’; was the president of the Society for Free Radical Research International (SFRRI). Helmut Sies introduced the concept of “Oxidative Stress” in 1985, and was the first to reveal hydrogen peroxide as a normal constituent of aerobic cell metabolism. His research interests comprise redox biology, oxidants, antioxidants, micronutrients.