An Optimized Expression Of Recombinant Hepcidin: Using Pichia Pastoris

Rashid, Farzana; Ijaz Ali, Dr; Rashid, Farzana; Ijaz Ali, Dr

ISBN 10: 3639283244 / ISBN 13: 9783639283242

Published by Vdm Verlag Dr. Müller, 2010

About this Item

136 pages. 8.66x5.91x0.31 inches. In Stock.

Seller Inventory # __3639283244

Bibliographic Details

• Title: An Optimized Expression Of Recombinant Hepcidin: Using Pichia Pastoris
• Publisher: Vdm Verlag Dr. Müller
• Publication Date: 2010
• Language: English
• ISBN 10: 3639283244
• ISBN 13: 9783639283242
• Binding: Paperback
• Condition: Brand New
• Weight: 0.25 kilograms

About this title

Synopsis

Expression and purification of small peptides have always been problematic due to enzymatic degradation and many other technical problems. We report cloning and expression of a low molecular weight human antimicrobial peptide ?hepcidin' (Hepc, 20 amino acids) in pPIC9K transformed into P. pastoris GS115. The study reveals that active hepcidin peptide can be successfully expressed in this methylotrophic yeast. The BMMY medium was found to be optimal for the hepcidin protein expression and growth of the recombinant strains. Hepcidin protein expressed in recombinant strains was about 3 mg/L. Peptide expression was verified by Western blotting and ELISA assay. Recombinant hepc 20 was purified through Reverse-Phase HPLC column and characterized by Mass Spectrometry and amino acid sequencing. It also exhibited antibacterial activity against Staphylococcus aureus and Bacillus subtilis.

About the Author

Dr Farzana Rashid is Assistant Professor at the Lahore University. She has 14 research publications, nine international and four national on her credit in the field of biotechnology, microbiology and education.Dr. Farzana has experience in coordinating Teacher Education programs and her interest areas include education and research.