2D PAGE: Sample Preparation and Fractionation: Volume 2 (Methods in Molecular Biology, 425)

Focusing on two-dimensional electrophoresis (2-DE), this book sets forth the principles and recent developments of sample preparation and fractionation tools in expression proteomics. 2-DE, with its unique capacity to resolve thousands of proteins in a single run, remains a fundamental research tool for nearly all protein-related scientific projects. Step-by-step instructions alongside detailed illustrations enable biochemists, molecular and cellular biologists, and protein scientists to quickly learn how to perform the methods. Tricks of the trade and notes on troubleshooting and avoiding pitfalls help ensure successful results every time. The methods covered in this book are not limited to 2-DE; they can also be applied to a broad range of protein separation techniques.

The complex nature of proteins often requires a multitude of sample preparation options. In 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics and specifically for two-dimensional electrophoresis (2-DE). Detailed laboratory methods describe the process of sample fractionation used to reduce the complexity of the protein sample and the methods of 2-DE analysis and a broad variety of gel-free mass spectrometry-based technologies to detect previously undetectable proteins. Vital information regarding protein isolation and preparation for reproducible proteome analysis previously overlooked by scientists has been clearly laid out in this pioneering two-volume set. Volume 2 illuminates specific applications of sample preparation tools and fractionation strategies to study a variety of biological systems.