The polymerase chain reaction (PCR) is now the standard method for amplifying nucleic acids and continues to find many new applications in basic, applied, and clinical research. Comprehensively updated and revised, this second edition of the best-selling "PCR" is designed as an introduction to the technique with authoritative coverage of a wide range of current applications. It includes current PCR applications such as genome mapping, pathogen detection, and quantitative PCR. The appendices include a comprehensive glossary of terms and listings of suppliers of instruments, reagents and consumables. From reviews of the first edition: "a very useful text and reference for new and experienced PCR users, I would rate it well above other recent texts that I have examined on the subject."

-- Trends in Cell Biology "This is a highly readable volume that could profitably be read by anyone about to employ PCR techniques, or who simply requires a basic understanding of the many manifestations of in vitro amplification." -- SGM Quarterly "the authors have succeeded in providing a viable introduction to PCR for researchers who are new to the technique, and also additional useful information for the veteran user. The balance between simple basic information and more specialized techniques is achieved making this a very useful book." -- TIBTECH

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